This new SD region of O. luzonensis maps between eyeless and you may Making Friends dating service 171M23F for the LG several (Tanaka mais aussi al. 2007). I performed subsequent linkage analysis and gotten a few men recombinants getting this place. One male had a good recombination breakpoint between OluX2-8 and you will OluX2-twenty five, and almost every other had good breakpoint ranging from OluX3-34 and you can OluX4-six, refining the newest SD part so you’re able to between OluX2-8 and OluX4-6 ( Figure 1A ).
(A) Genetic and you will real map of your own sex-choosing countries towards X and Y chromosomes. step 1, normal XY men; dos, XX females; step three and you will cuatro, recombinant male. Blue column, countries produced from new Y-chromosome; purple, from the X chromosome. Red-bar, BAC clones; bluish club, fosmid clones. Black colored arrows suggest forecast family genes. (B) RT–PCR circumstances from PGs (PG1–9) regarding XX and XY body trunk from the 0 days shortly after hatching. M, size marker.
We constructed a beneficial BAC collection away from a keen XX fish and a great fosmid collection regarding a beneficial YY fish, therefore generated actual maps of SD region of the X and you can Y chromosomes. This particular area try wrapped in a couple of BAC clones (OluBXKN2 and you will OluBXKN1) on X chromosome with eight fosmid clones (OluFYstep 13-step one, OluFY24-1, OluFYstep 18-step one, OluFY3-1, OluFY8-step one, OluFY7-step one, and OluFY29-1) for the Y chromosome ( Profile 1A ). The whole nucleotide series is dependent on playing with shotgun sequencing, with the exception of a repeated part from inside the OluFY3-step one and OluBXKN1. Limit investigation of each other clones shown the amount of the latest repeated region was a similar for chromosomes (data maybe not found). Fortsett å lese Nine genes was predict on SD area