Locations of Platanthera chlorantha (PS1 and PS2, PB1–PB4, circles) and you will Cephalanthera rubra (CK1 and you will CK2, CB1–CB7, triangles) communities inside north-east Poland.
Analysis urban area and you may sampling
I investigated half dozen P. chlorantha and you will 9 C. rubra populations within the northern-eastern Poland (Bialowieza and Knyszynska Primeval Forest, Szeszupa lake valley) within the natural, semi-absolute and you can anthropogenic teams of federal and you may land areas, supplies and protected section, such Natura 2000 internet ( Fig. 1). Though he could be located in protected section, of several exists to the train embankments, along roads and you can routes inside the forests or in clearings.
This new testing procedure relied towards the population size. Leaf examples away from most ramets within this populations each and every types have been removed (except people PS2; Dining table step 1); no examples had been extracted from busted otherwise really more youthful anybody. One hundred and ninety-eight trials from P. chlorantha and you may 95 trials from C. rubra had been compiled. Leaf muscle was kept on ice up until it may be kept from the ?80 °C, pending allozyme investigation. All collected trials were used to possess allozyme study.
N, population size; NS, number of samples analysed; NGrams/NW, number of generative ramets/number of vegetative ramets; PPOL, percentage of polymorphic loci; A, mean number of alleles per locus; HO, observed heterozygosity; HE, expected heterozygosity; FIs actually, inbreeding coefficient; G, number of genotypes, G/NS, clonal diversity; GU, number of unique genotypes; GU %, percentage of unique genotypes (Fischer’s exact test: P < 0.05, **P < 0.01, ***P < 0.001); #, sum of parameters. Fortsett å lese The new dating ranging from parameters from hereditary (P